Accession Number : ADA299344

Title :   Mapping of a Breast Carcinoma Tumor Suppressor Gene to Chromosome 11p15.5.

Descriptive Note : Annual rept. 1 Jul 94-30 Jun 95,

Corporate Author : NORTH CAROLINA UNIV AT CHAPEL HILL

Personal Author(s) : Gasque-Carter, Perry ; Weissman, Bernard E.

PDF Url : ADA299344

Report Date : 26 JUL 1995

Pagination or Media Count : 10

Abstract : The identification of human tumor suppressor genes has led to new insights into the mechanisms of human cancer development. Most of the known tumor suppressor genes were isolated by determining their chromosomal location using molecular markers or cytogenetics. By this manner, several reports have mapped a tumor suppressor gene for human breast cancer to a region of chromosome l1p15.5. Our preliminary physical map of this area suggests that this region contains less than 1000 kilobases (kb). During the last year, we have gathered cosmids, P1s and yeast artificial chromosomes (YACs) from this area for the isolation of new polymorphic markers. We have also obtained matched DNA samples from over 25 normal breast and breast tumors during the past year. During the next funding period, we plan to initiate the identification of new di- tri- and tetra-nucleotide repeats from our genomic inserts in the region. We will also start loss of heterozygosity studies to further narrow the tumor suppressor region. The availability of a novel tumor suppressor gene inactivated in 25% of breast cancers would lead to new methods for treatment and early detection and may provide important clues about the process of normal mammalian tissue development

Descriptors :   *NEOPLASMS, *CHROMOSOMES, *GENES, *CANCER, *MAMMARY GLANDS, *SUPPRESSORS, POSITION(LOCATION), DETECTION, HUMANS, MOLECULES, ISOLATION, DEOXYRIBONUCLEIC ACIDS, REGIONS, SAMPLING, MAPS, YEASTS, MARKERS, MATCHING, GENETICS, POLYMORPHISM, CYTOLOGY.

Subject Categories : Anatomy and Physiology
      Genetic Engineering and Molecular Biology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE