Accession Number : ADA299442

Title :   Effects of Lyophilization on Metabolic Integrity of Red Blood Cells.

Descriptive Note : Progress quarterly rept. no. 3, 1 Sep 31 Dec 94,

Corporate Author : COLORADO UNIV HEALTH SCIENCES CENTER DENVER

Personal Author(s) : Carpenter, John F.

PDF Url : ADA299442

Report Date : 31 DEC 1994

Pagination or Media Count : 3

Abstract : The column needed for the HPLC assay of adenylates was changed to an aminopropyl column, when it was discovered that the old reverse phase column had to be cleaned extensively after every 4-5 biological samples were assayed. A new protocol for the aminopropyl column has been identified. With this protocol and column, we should be able to run at least 400-500 samples before having to clean the column. Thus, our overall efficiency will be greatly improved. The HPLC assay for glycolytic intermediates has been improved to resolve two overlapping peaks for key metabolites. This approach, plus installing a larger sample loop, should eliminate the need for fractionating samples prior to running the assay. A separate column and protocol for measuring the triosephosphate glycolytic intermediates has been identified, which should allow for guantitation of these previously difficult to assess compounds.

Descriptors :   *BLOOD CELLS, *LYOPHILIZATION, METABOLISM, REVERSIBLE, SAMPLING, ERYTHROCYTES, METABOLITES, FRACTIONATION.

Subject Categories : Anatomy and Physiology
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE