Accession Number : ADA299460

Title :   Generation of Novel Protein Receptors.

Descriptive Note : Final rept. 1 Dec 93-30 Nov 94,

Corporate Author : SCRIPPS RESEARCH INST LA JOLLA CA

Personal Author(s) : Hilvert, Donald

PDF Url : ADA299460

Report Date : 30 NOV 1994

Pagination or Media Count : 4

Abstract : Families of protein receptors will be created from a small, stable template. The experimental approach involves synthesizing the gene for the template protein and cloning it into a bacteriophage vector to allow phage display of the protein. In this way, replication and recognition can be coupled. Repeated rounds of mutagenesis and affinity chromatography will be employed to select the desired binding properties out of the diverse population created from the original protein scaffold.

Descriptors :   *PROTEINS, *GENETIC ENGINEERING, *RECEPTOR SITES(PHYSIOLOGY), *BACTERIOPHAGES, STABILITY, EXPERIMENTAL DATA, POPULATION, DISPLAY SYSTEMS, TEMPLATES, CLONES, MUTAGENS, CHROMATOGRAPHY.

Subject Categories : Medicine and Medical Research
      Genetic Engineering and Molecular Biology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE