Accession Number : ADA299494

Title :   Detection and Characterization of Autoantigens in Breast Cancer.

Descriptive Note : Annual rept. 15 Jul 94-14 Jul 95,

Corporate Author : MONTEFIORE HOSPITAL AND MEDICAL CENTER BRONX NY

Personal Author(s) : Racevskis, Janis

PDF Url : ADA299494

Report Date : 09 AUG 1995

Pagination or Media Count : 10

Abstract : By screening cDNA libraries from primary ductal breast carcinomas with autologous patient serum, we have detected and isolated three immunoreactive cDNA clones. One of the isolated breast tumor autoantigens (Ngp-1), encodes a GTP-binding protein which belongs to a newly described subfamily of GTpases. Immunohistochemical analysis of tissue sections revealed that the antigen was exclusively localized in the nucleolus and nucleolar organizer regions in all cell types analyzed. The second autoantigen isolate (Auag2) is also a newly discovered gene product which is not expressed in transformed breast tumor epithelial cell lines, but is however detectable in breast tumors. The sequence of AuAg2 bears a partial homology to human heparin-binding angiogenic vascular endothelial growth factor. The third potential isolate remains to be characterized, and more cDNAs are being screened with breast cancer patient serum to identify additional autoantigens.

Descriptors :   *NEOPLASMS, *ANTIGENS, *CANCER, *MAMMARY GLANDS, DETECTION, CELLS, ISOLATION, GENES, CLONES, PATIENTS, BLOOD SERUM, IMMUNOCHEMISTRY, HISTOCHEMISTRY.

Subject Categories : Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE