Accession Number : ADA299495

Title :   Molecular Diagnosis for Breast Malignancy.

Descriptive Note : Annual rept. 1 Jul 94-30 Jun 95,

Corporate Author : GEORGETOWN UNIV WASHINGTON DC

Personal Author(s) : Chen, Wen-Tien

PDF Url : ADA299495

Report Date : 28 JUL 1995

Pagination or Media Count : 10

Abstract : This grant investigates a major surface protease complex of malignantly transformed cells (malignancy antigens), containing maligrin and seprase, as indicators of the metastatic potential of breast cancer cells. The aims are to evaluate conceivable invasion-related molecules as prognostic markers for node-negative breast cancer. During the first year of this grant, we have identified the p29 maligrin-associated protein and its corresponding cDNA, selected monoclonal antibodies useful in staining of paraffin sections, and established monoclonal antibody sandwich ELISA technique using breast cancer cells in culture (6 cell lines) as proposed in the Statement of Work. MDA-MB-231 cDNA library was initially screened with anti-p29 antibodies. Positive clones were sequenced and identified as a 58-kDa human tumor derived collagenase-stimulating factor, now called EMMPRfN. The p29 antigen was originated from bovine semm as identified by the sandwich ELISA, and it bound to cancer cell surface invadopodia. Future isolation and expression of EMMPRfN will be sought to test the recombinant molecule in stimulating production of

Descriptors :   *DIAGNOSIS(MEDICINE), *MONOCLONAL ANTIBODIES, *ANTIGENS, *CANCER, *MAMMARY GLANDS, PRODUCTION, MOLECULES, CELLS, ENZYMES, ISOLATION, NEOPLASMS, DEOXYRIBONUCLEIC ACIDS, SURFACES, DIAGNOSIS(GENERAL), CLONES, MARKERS, CELLS(BIOLOGY), IMMUNOASSAY, INDICATORS, SANDWICH CONSTRUCTION, PEPTIDE HYDROLASES, METASTASIS.

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE