Accession Number : ADA299550

Title :   Preclinical Investigation of Lyophilized Platelet Preparations.

Descriptive Note : Triannual rept. no. 1, 1 Sep-31 Dec 94,

Corporate Author : EAST CAROLINA UNIV SCHOOL OF MEDICINE GREENVILLE NC

Personal Author(s) : Bode, Arthur P. ; Read, Marjorie S.

PDF Url : ADA299550

Report Date : 24 FEB 1995

Pagination or Media Count : 11

Abstract : Preparations are underway to perform initial studies in the second in vivo model of hemostasis involving hemostasis support of pigs after inducing a massive abdominal bleed by a tear in the aorta. We have begun making lyophilized platelet preparations from donor-pigs and have performed initial integrity studies on rehydrated aliquots of the final product. The protocol developed for stabilizing and lyophilizing human platelets was used without modification with success. Our first two trials of these preparations in the bleeding pig model were carried out in the time period between Dec. 31 and the date of this writing and will be presented in the next reporting period. We have prepared and stockpiled enough lyophilized pig platelets at this point to run 3 more trials, but will continue to make more pig platelet preparations whenever donor animals become available at our institution. Current plans are to perform at least 12 trials, with controls, over the next six months.We have continued to perform analysis of hemostatic potential of rehydrated human, dog, or pig platelets in the OSA.In the period of this report, we tested 19 different platelet preparations along with an equal number of controls of fresh or stored blood bank platelets. In general the results have shown that the PARA2l-type human lyophilized platelets show the best performance compared to PARA22 or PARA45-type platelets. The dog and pig rehydrated platelets have given variable results, which may have been due to interactions of plasma and cellular components in the reconstitution of whole blood for testing. We have found that pig or dog RBC cannot be used in combination with human plasma to test pig or dog platelet preparations; however, favorable results were obtained with pig or dog platelets resuspended in human plasma combined with washed human RBC.

Descriptors :   *IN VIVO ANALYSIS, *BLOOD PLATELETS, *LYOPHILIZATION, TEST AND EVALUATION, CONTROL, PREPARATION, HUMANS, PLASMAS(PHYSICS), CLINICAL MEDICINE, VARIABLES, ANIMALS, CELLS(BIOLOGY), DOGS, BLOOD CIRCULATION, AORTA, BLOOD PLASMA, HEMORRHAGE, SWINE, VASOCONSTRICTING, BLOOD STORAGE, CONSTRICTIONS.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE