Accession Number : ADA299744

Title :   WNT-5a and WNT-4 Regulates Cell Growth in C57MG Mammary Epithelial Cells.

Descriptive Note : Annual rept. 1 Jul 94-30 Jun 95,

Corporate Author : MILTON S HERSHEY MEDICAL CENTER PA

Personal Author(s) : Olson, Daniel J.

PDF Url : ADA299744

Report Date : 30 JUL 1995

Pagination or Media Count : 16

Abstract : The implications for determining whether wnt-5a and wnt-4 act as mediators of normal cell growth are relevant to the detection, diagnosis, and the treatment of breast cancer. That is, it is important ultimately to understand whether the inappropriate down-regulation of certain wnt-genes that are spatially/temporally expressed in developing mammary glands, such as wnt-5a and wnt-4, leads to loss of growth control. C57MG mammary epithelial cells have recently been found to endogenously express wnt-5a and wnt-4 whose RNA levels are reduced in the presence wnt-gene family members which transform this cell line. The intent of this proposal has been to develop new cell lines by transfecting C57MG cells with DNA wnt-5a and/or wnt-4 constructs which express antisense RNA to determine whether cell transformation will occur. In conjunction with this, new cell lines were also to be formulated by over-expressing wnt-5a and/or wnt-4 in an attempt to revert an already transformed phenotype. Preliminary results found are consistent with the importance for normal wnt-5a and/or RNA transcripts levels in directing C57MG mammary epithelial cell phenotype, including cell proliferation and transformation.

Descriptors :   *EPITHELIUM, *CANCER, *MAMMARY GLANDS, *GROWTH(PHYSIOLOGY), DETECTION, GROWTH(GENERAL), DEOXYRIBONUCLEIC ACIDS, DIAGNOSIS(MEDICINE), CELLS(BIOLOGY), RIBONUCLEIC ACIDS, TRANSFORMATIONS.

Subject Categories : Medicine and Medical Research
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE