Accession Number : ADA299841

Title :   Sensitivity Increase in Nicotinic Acetylcholine Receptor Binding in Fiber Optic Wave Guide.

Descriptive Note : Final rept. Jun-Sep 92,

Corporate Author : EDGEWOOD RESEARCH DEVELOPMENT AND ENGINEERING CENTER ABERDEEN PROVING GROUND MD

Personal Author(s) : Menking, Darrel E. ; Thompson, Roy G. ; Heitz, Jonathon M. ; Rogers, Kim R.

PDF Url : ADA299841

Report Date : SEP 1995

Pagination or Media Count : 16

Abstract : Ligand binding studies with nicotinic acetylcholine receptor on the fiber optic biosensor indicate K (sub i) values for antagonists were similar to those obtained in membrane binding assays. The agonists appear much less potent. This may be due to the inability of the receptor to make appropriate conformational changes necessary for agonist binding. Spacer arms may give the receptor the conformational freedom required. To test whether the spacer arms increase the conformational freedom of the immobilized receptors, receptors were tethered to the surface of the fiber with the antibodies specific for the receptor but did not block the binding sites of the receptor. Receptor binding of agonists on tethered receptors was at least one order of magnitude lower than values previously reported.

Descriptors :   *FIBER OPTICS, *WAVEGUIDES, *ACETYLCHOLINE, *NICOTINE, DETECTORS, SENSITIVITY, LIGANDS, MEMBRANES, ANTIBODIES, BIOLOGICAL DETECTION, BINDERS, ASSAYING, SENSE ORGANS, CHEMORECEPTORS, SPACERS.

Subject Categories : Pharmacology
      Biology
      Organic Chemistry
      Fiber Optics and Integrated Optics

Distribution Statement : APPROVED FOR PUBLIC RELEASE