Accession Number : ADA299909

Title :   Electrostatic Control of Acetylcholinesterase Reactivity.

Descriptive Note : Final technical rept. 1 Apr 89-31 Dec 94,

Corporate Author : STATE UNIV OF NEW YORK AT BUFFALO DEPT OF BIOCHEMICAL PHARMACOLOGY

Personal Author(s) : Berman, Harvey A.

PDF Url : ADA299909

Report Date : 01 JUL 1995

Pagination or Media Count : 3

Abstract : These studies examined chiral reactivity, the magnitude and importance of steric interactions, and the importance of the electrostatic charge distribution in governing reactivity of acetylcholinesterase (AchE). To do this, we employed a comprehensive series of resolved enantiomeric methylphosphonates, a comprehensive series of alkylphosphonates, and fluorescent methylphosphonates.As a complementary index to detailed stopped-flow analysis of inhibition kinetics, we monitored kinetics of aging and oxime reactivation. Together with equilibrium binding studies, employing the reversible fluorescent ligand decidium diiodide, these provided an independent index of ligand orientation achieved by the organophosphonates within the enzyme active center.

Descriptors :   *ACETYLCHOLINESTERASE, *ELECTROSTATICS, CONTROL, FLUORESCENCE, AGING(MATERIALS), SPECTROSCOPY, DISTRIBUTION, ENZYMES, ORIENTATION(DIRECTION), REACTIVITIES, EQUILIBRIUM(GENERAL), LIGANDS, INDEXES, KINETICS, INHIBITION, PHOSPHONATES, ELECTROSTATIC CHARGE, ORGANIC PHOSPHORUS COMPOUNDS, ORGANOPHOSPHATES, OXIMES.

Subject Categories : Biochemistry
      Electricity and Magnetism

Distribution Statement : APPROVED FOR PUBLIC RELEASE