Accession Number : ADA299925

Title :   Progress Report for Contract N00014-94-C-0015 (AT and T Bell Lab).

Descriptive Note : Progress rept.,

Corporate Author : AT AND T BELL LABS MURRAY HILL NJ

Personal Author(s) : Yuste, Rafael ; Tank, David W.

PDF Url : ADA299925

Report Date : 22 FEB 1995

Pagination or Media Count : 5

Abstract : Our main goal for the next year is to perform optical recordings with single-cell resolution of a population of neurons in the adult cortical slice These experiments did not worked initially because of the failure of calcium indicators to label populations of adult neurons. Nevertheless, preliminary experiments by us and a number of groups suggest that extracellular injections of dextrans coupled to calcium indicators can be used to labeled populations of neurons in a variety of Systems. Other possible approaches are the use of calcium indicators with less acetoxymethyl esters or the use of a new generation of voltage-sensitive dyes that can be transported retrogradely or that have much larger signals that previous ones. These different strategies appear to us very promising solutions to the problem.

Descriptors :   *OPTICAL PROPERTIES, *NERVE CELLS, *INJECTIONS(MEDICINE), CALCIUM, RESOLUTION, FAILURE, POPULATION, PROBLEM SOLVING, RECORDING SYSTEMS, ESTERS, CELLS(BIOLOGY), INDICATORS, ADULTS.

Subject Categories : Anatomy and Physiology
      Optics

Distribution Statement : APPROVED FOR PUBLIC RELEASE