Accession Number : ADA300016

Title :   Targeted Gene Delivery to Accomplish Gene Therapy for Breast Cancer.

Descriptive Note : Annual rept. 1 Aug 94-31 Jul 95,

Corporate Author : ALABAMA UNIV IN BIRMINGHAM

Personal Author(s) : Curiel, David T.

PDF Url : ADA300016

Report Date : 28 AUG 1995

Pagination or Media Count : 13

Abstract : We are developing methods to derive a gene transfer vector capable of accomplishing targeted gene delivery to metastatic breast cancer cells. In this regard, strategies were employed to modify adenoviral vectors by altering their binding tropism. Genetic methods undertaken allowed for the modification of the native adenoviral binding protein (fiber) to incorporate cancer-relevant cell-binding ligands. Ittrnunologic methods yielded an anti fiber antibody which specifically ablates native adenoviral tropism and provides a site for the subsequent addition of breast cancer-relevant ligands. Chemical methods demonstrated that biotin could be added to the virion exterior as an anchor for a streptavidin bridge to allow for incorporating of breast cancer relevant ligands. The results developed herein have allowed for the successful modification of the adenoviral vector to allow it to accomplish cell-specific gene delivery. This important first step will now allow the evaluation of this vector system in the context of in vitro and in vivo models of breast cancer. The utility of the vector in this context will allow the development of gene therapy strategies for disseminated breast cancer on this basis.

Descriptors :   *GENES, *GENETICS, *CANCER, *MAMMARY GLANDS, *METASTASIS, FIBERS, STRATEGY, CELLS, IN VITRO ANALYSIS, CHEMISTRY, VECTOR ANALYSIS, THERAPY, ANTIBODIES, TRANSFER, IN VIVO ANALYSIS, VITAMIN B COMPLEX.

Subject Categories : Medicine and Medical Research
      Genetic Engineering and Molecular Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE