Accession Number : ADA300046

Title :   Molecular Determinants of Antiestrogen and Drug Sensitivity in Breast Carcinoma Cells.

Descriptive Note : Annual rept. 15 Jul 94-14 Jul 95,

Corporate Author : ILLINOIS UNIV AT CHICAGO CIRCLE

Personal Author(s) : Roninson, Igor B.

PDF Url : ADA300046

Report Date : 14 AUG 1995

Pagination or Media Count : 20

Abstract : NCF7 breast carcinoma cells were rendered susceptible to infection with recombinant ecotropic retroviruses, through transfection with the gene for murine ecotropic receptor and by testing individual transfectants for infectability. A genetic suppressor element (GSE), derived from the BCL2 gene which inhibits active cell death (apoptosis), was introduced into NCF7 cells. The BCL2-derived GSE sensitized MCF7 cells to different chemotherapeutic drugs, indicating that BCLt2 is an important determinant of chemoresistance of these cells. A retroviral library of normalized cDNA fragments, derived from Heta cell line, was introduced into NCF7 cells, and selection of GSEs that render MCF7 cells resistant to doxorubicin was initiated. A normalized cDNA library is also being prepared from NCF7 cells, for use as a source of GSEs conferring resistance to tamoxifen.

Descriptors :   *CELLS(BIOLOGY), *CHEMOTHERAPY, *CANCER, *MAMMARY GLANDS, *ESTROGENS, NEOPLASMS, SENSITIVITY, INFECTIOUS DISEASES, DRUGS, DEATH, GENETICS, SUPPRESSORS, RETROVIRUSES.

Subject Categories : Medicine and Medical Research
      Anatomy and Physiology
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE