Accession Number : ADA300081

Title :   Role of Integrin-Related Tyrosine Kinases in Growth Control of Normal and Tumorigenic Human Mammary Epithelium.

Descriptive Note : Annual rept. 1 Jul 94-30 Jun 95,

Corporate Author : SCRIPPS RESEARCH INST LA JOLLA CA

Personal Author(s) : Plopper, George ; Quaranta, Vito

PDF Url : ADA300081

Report Date : 30 JUL 1995

Pagination or Media Count : 39

Abstract : The purpose of this study is to identify integrin-specific signalling events that may be responsible for development of the malignant pheno type in mammary epithelium. Our laboratory has focused on a novel ligand for epithelial integrins, laminin-5r, which is located in mammary epithelial basement membrane. We hypothesize that binding of integrins to laminin-5r elicits downstream signalling events that may help define the normal phenotype of mammary epithelial cells; disruption of these signals may contribute to the development of malignancy. To date we have succeeded in raising six monoclonal anti-bodies against laminin-5r, and have used them to define distinct structural conformations of laminin-5r using a combination of immuno-precipitation, western blot, and elisa assays. Also, we have used these antibodies to affinity purify laminin-5r from conditioned medium of a rat epithelial cell line, 804G. Finally, we have shown that one of these antibodies blocks adhesion of a variety of cells, including mammary epithelial cells, to purified laminin-5r.

Descriptors :   *GROWTH(GENERAL), *CANCER, *MAMMARY GLANDS, CONTROL, EPITHELIUM, RATS, CELLS, ENZYMES, PRECIPITATION, SIGNALS, MONOCLONAL ANTIBODIES, MEDICAL SERVICES, IMMUNOASSAY, ASSAYING.

Subject Categories : Medicine and Medical Research
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE