Accession Number : ADA300169

Title :   Effect of Hormone Replacement Therapies and Dietary Phytoestrogens on the Mammary Gland of Macaques.

Descriptive Note : Annual rept. 1 Jul 94-30 Jun 95,

Corporate Author : BOWMAN GRAY SCHOOL OF MEDICINE WINSTON-SALEM NC

Personal Author(s) : Cline, J. M.

PDF Url : ADA300169

Report Date : 27 JUL 1995

Pagination or Media Count : 55

Abstract : The purpose of this study was to use histomorphometric and immunohistochemical techniques to study the incidence and characteristics of mammary gland hyperplasia, dysplasia and other possible indicators of breast cancer risk, in cynomolgus macaques given long-term hormonal treatments. Treatments evaluated to date include conjugated estrogens (CEE), medroxyprogesterone acetate (MPA), the combination of CEE and MPA, tamoxifen, estradiol (E2), and 17a-dihydroequilenin (DHEN). Pathologic evaluation, histomorphometric evaluations, and staining for estrogen receptor, progesterone receptor, and the proliferation marker Ki-67 MIB were done. Results are as follows: The addition of MPA to CEE therapy increases, rather than decreases, mammary gland proliferation. This finding is in contrast to the uterus, where MPA antagonizes the proliferation induced by CEE. Estrogen receptor expression in mammary gland epithelium is decreased by treatment with CEE, and further decreased by the addition of MPA. Progesterone receptor expression in mammary gland epithelium is increased by treatment with CEE, and decreased by the addition of MPA. Tamoxifen treatment does not induce mammary land proliferation beyond that seen in controls; this is in contrast to a marked uterotrophic effect. DHEN does not induce mammary gland or endometrial proliferation, relative to controls and in contrast to E2.

Descriptors :   *HORMONES, *REPLACEMENT, *MAMMARY GLANDS, *PROGESTERONE, *ESTROGENS, TEST AND EVALUATION, CONTROL, CONTRAST, EPITHELIUM, LONG RANGE(TIME), THERAPY, PATHOLOGY, IMMUNOLOGY, INDICATORS, SENSE ORGANS, HISTOLOGY, DYSPLASIA, MACAQUE MONKEYS, UTERUS.

Subject Categories : Medicine and Medical Research
      Anatomy and Physiology
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE