Accession Number : ADA300474

Title :   Characterization of CTL Recognized Epitodes on Human Breast Tumors.

Descriptive Note : Annual rept. 19 Aug 94-18 Aug 95,

Corporate Author : TEXAS UNIV AT HOUSTON

Personal Author(s) : Ioannides, Constantin G.

PDF Url : ADA300474

Report Date : 18 SEP 1995

Pagination or Media Count : 138

Abstract : The overall objective is to develop novel therapeutic approaches to breast cancer by understanding the molecular and cellular basis of HER-2 recognition on breast tumor cells by cytotoxic T lymphocytes (CTL). To optimize CTL recognition of antigenic epitopes on HER-2 we defined the role of residues in the epitopes in enhancing HLA-binding and target lysis. We found that in the HLA-A2 system positions PI - P3 are more amenable to changes which stabilize binding and do not affect CTL recognition. We are now characterizing tumor-bound peptides through immuno-affinity elution, HPLC, and defining peptides that can reconstitute common Ag which may be clinically relevant. To address the nature and frequency of ex vivo CTL responses to breast tumors and HER-2, we are developing CD8+ CTL lines and clones from lymphocytes infiltrating primary or metastatic breast tumors and defining their specificity of Ag recognition in functional assays.

Descriptors :   *CYTOTOXINS, *T LYMPHOCYTES, *LYMPHOCYTES, *MAMMARY GLANDS, *BREAST CANCER, HUMANS, PEPTIDES, NEOPLASMS, RESPONSE, THERAPY, CLONES, CELLS(BIOLOGY), RESIDUES, METASTASIS.

Subject Categories : Biology
      Medicine and Medical Research
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE