Accession Number : ADA300477

Title :   Identification of Biomarkers in Bacteria by Pyrolysis - Tandem Mass Spectrometry.

Descriptive Note : Final rept.,

Corporate Author : COLORADO SCHOOL OF MINES GOLDEN

Personal Author(s) : Voorhees, Kent J.

PDF Url : ADA300477

Report Date : 25 SEP 1995

Pagination or Media Count : 150

Abstract : The use of the biomarkers for the cLassification and identification of bacteria is discussed. ResuLts on the pyrolysis-mass spectrometry of proteins, peptides, and fatty acids are described. In situ chemicaL reactions of fatty acids and fatty acid precursors using tetramethyl ammonium hydroxide produced fatty acid methyl ester FADIE) distributions similar to those obtained from cLassical derivatization procedures. The FAME mass spectrometry data allowed for differentiation of Gram-positive and negative bacteria. Moreover, pathogenic bacteria were identified based on their FAME profiles generated using in situ derivatization in the Army's Chemical and Biological Mass Spectrometer. Three peer-reviewed papers and eleven oral presentations have resulted from the research.

Descriptors :   *BACTERIA, *MASS SPECTROMETRY, METHYL RADICALS, PEPTIDES, PRECURSORS, PROTEINS, CHEMICAL REACTIONS, AMMONIUM COMPOUNDS, MARKERS, HYDROXIDES, PATHOGENIC MICROORGANISMS, PYROLYSIS, TRACER STUDIES, FATTY ACID ESTERS.

Subject Categories : Medicine and Medical Research
      Nuclear Physics & Elementary Particle Physics

Distribution Statement : APPROVED FOR PUBLIC RELEASE