Accession Number : ADA300480

Title :   Novel Approaches to the Characterization of Specific Protein-Protein Interactions Important in Gene Expression.

Descriptive Note : Final rept. 15 Aug 91-14 Aug 95,

Corporate Author : PURDUE RESEARCH FOUNDATION LAFAYETTE IN DIV OF SPONSORED PROGRAMS

Personal Author(s) : Somerville, Ronald L.

PDF Url : ADA300480

Report Date : 14 OCT 1994

Pagination or Media Count : 7

Abstract : Through the application of a range of biochemical, immunochemical and genetic techniques, we have accomplished the following goals: First, we characterized a previously undescribed protein, WrbA and showed that it functions as an accessory protein in the binding of TrrR to its operator target; second, we demonstrated that WrbA is a stationary-phase protein, and that the regulation of promoters controlled by TrpR becomes more stringent in stationary phase cells; third, we developed a method for the purification of the TyrR protein, a dual-specificity transcription factor that can both activate and inhibit the action of RNA polymerase. The domain structure of TyrR was established by chemical procedures.

Descriptors :   *PROTEINS, INTERACTIONS, POLYMERIZATION, TARGETS, ESCHERICHIA, PURIFICATION, GENES, OPERATORS(PERSONNEL), CELLS(BIOLOGY), RIBONUCLEIC ACIDS, GENETICS, CHEMICAL ENGINEERING.

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE