Accession Number : ADA300539

Title :   Progress Report for Contract Number N00014-93-C-0019. Quarterly Report Nomber 8. (Hawaii Biotechnology Group, Inc.).

Descriptive Note : Quarterly rept. no. 8, 1 Oct-31 Dec 94.

Corporate Author : HAWAII APPLIED RESEARCH INC HONOLULU

PDF Url : ADA300539

Report Date : 31 DEC 1994

Pagination or Media Count : 28

Abstract : (1) The production of anti-ferret IgA antibodies. Status: Immunization of rabbits and collection of sera completed. Mouse ascites successfully cross-adsorbed; material sent to NMRI researchers in 12/94. (2) The purification of lipopolysaccharide from Sbigella and Campylobacter species. Status: Shigella sonnei and S. flexneri LPS delivered to NMRI researchers. Purification of C. jejuni LPS reinstated. Have collected 173mg of required a 200mg LPS. Production of remainder in progress. (3) The development of an enzyme immunoassay for the detection of enteroaggregative Escherichia coli heat-stable toxin. Status: Initial recombinants provided by Navy unsatisfactory. Work suspended in 10/93 due to contract funding reduction. Suitable recombinants still not available. (4) The production of monoclonal antibodies against strain-specific antigenic epitopes on Campylobacter coli flagella. Status: Completed 3 fusions. No strain specific MAbs identified.

Descriptors :   *ANTIBODIES, *MONOCLONAL ANTIBODIES, *BIOTECHNOLOGY, *TOXINS AND ANTITOXINS, *SHIGELLA SONNEI, DETECTION, CONTRACTS, PRODUCTION, ENZYMES, THERMAL STABILITY, ESCHERICHIA COLI, REDUCTION, PURIFICATION, STRAINS(BIOLOGY), MICE, IMMUNOASSAY, HAWAII, IMMUNIZATION, SPIRILLACEAE, MONEY, RABBITS, LIPOPOLYSACCHARIDES, ASCITES.

Subject Categories : Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE