Accession Number : ADA300572

Title :   Novel Approaches to the Characterization of Specific Protein-Protein Interactions Important in Gene Expression.

Descriptive Note : Final technical rept.,

Corporate Author : PURDUE RESEARCH FOUNDATION LAFAYETTE IN DIV OF SPONSORED PROGRAMS

Personal Author(s) : Somerville, Ronald L.

PDF Url : ADA300572

Report Date : 14 OCT 1995

Pagination or Media Count : 7

Abstract : Through the application of a range of biochemical, immunochemical and genetic techniques, we have accomplished the following goals: First, we characterized a previously undescribed protein, WrbA and showed that it functions as an accessory protein in the binding of TrpR to its operator target; second, we demonstrated that WrbA is a stationary-phase protein, and that the regulation of promoters controlled by TrpR becomes more stringent in stationary phase cells; third, we developed a method for the purification of the TyrR protein, a dual-specificity transcription factor that can both activate and inhibit the action of RNA polymerase. The domain structure of TyrR was established by chemical procedures.

Descriptors :   *PROTEINS, *GENES, *GENETICS, INTERACTIONS, CHEMICALS, POLYMERIZATION, TARGETS, ESCHERICHIA COLI, PURIFICATION, STATIONARY, OPERATORS(PERSONNEL), RIBONUCLEIC ACIDS, CHEMICAL ENGINEERING.

Subject Categories : Genetic Engineering and Molecular Biology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE