Accession Number : ADA300581

Title :   Programmed Cell Death in Breast Cancer.

Descriptive Note : Annual progress rept. 1 Oct 94-30 Sep 95,

Corporate Author : CASE WESTERN RESERVE UNIV CLEVELAND OH

Personal Author(s) : Distelhorst, Clark W.

PDF Url : ADA300581

Report Date : 28 AUG 1995

Pagination or Media Count : 16

Abstract : E HAVE INITIATED STUDIES OF PROGRAMMED CELL DEATH, OR POPTOSIS, IN HUMAN BREAST CANCER CELLS. Using the MCF-V reast cancer cell line, we have found that cell growth is stimulated by supplementing the culture medium with l%%-estradio1, and inhibited by charcoal stripping serum o remove steroid hormones. We have found that MCF-% cells are resistant to induction of apoptosis by the calcium pump inhibitorr thapsigargin, ostensibly due to expression of Bcl-2. We have found that estrogen ithdrawal decreases the level of Bcl-2 expression, but does not increase the susceptibility of cells to apoptosis due to constitutive Bcl-2 expression.

Descriptors :   *DEATH, *CELLS(BIOLOGY), *BREAST CANCER, CALCIUM, HORMONES, HUMANS, RESISTANCE, COMPUTER PROGRAMMING, PUMPS, INDUCTION SYSTEMS, MAMMARY GLANDS, CULTURE MEDIA, STEROIDS, ESTROGENS.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE