Accession Number : ADA301623

Title :   Tumor Suppressor Genes in Early Breast Cancer and Its Progression.

Descriptive Note : Annual rept. 1 Sep 94-31 Aug 95,

Corporate Author : WASHINGTON UNIV ST LOUIS MO SCHOOL OF MEDICINE

Personal Author(s) : Radford, Diane M.

PDF Url : ADA301623

Report Date : SEP 1995

Pagination or Media Count : 33

Abstract : Ductal carcinoma in situ (DCI S) of the breast is a pre-invasive form of breast cancer, which in some cases is a precursor to invasive ductal cancer. Many tumor suppressor loci are inactivated in invasive breast cancer, however little is known concerning the genetic events preceding invasion. Using polymerase chain reaction of microsatellite markers we have allelotyped 61 tumor/normal pairs from patients with DCIS. Tumor DNA template was obtained from microdissected archival samples. Significant loss of heterozygosity (LOll) was found for chromosomal loci on 8p (18.7%), 13q (18%), 16q (28.6%), 17p (37.5%) and 17q (15.9%). The region of allelic loss on 8p has been examined with an expanded panel of markers on 64 tumors, and the smallest region of deletion narrowed down to 1.3 cM. A fine structure map of 8p is being created using genotypic data from CEPH families. Assay of twelve sets of synchronous in situ and invasive breast cancer revealed that clonal expansion takes place in the progression of DCIS and that loci on lip may be involved on the invasive phenotype.

Descriptors :   *NEOPLASMS, *GENES, *MAMMARY GLANDS, *BREAST CANCER, POLYMERIZATION, PANELS, DEOXYRIBONUCLEIC ACIDS, SAMPLING, TEMPLATES, MARKERS, EXPANSION, ARCHIVES, GENETICS, CHAIN REACTIONS, SUPPRESSORS.

Subject Categories : Anatomy and Physiology
      Genetic Engineering and Molecular Biology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE