Accession Number : ADA301728

Title :   Breast Cancer in Ataxia Telangiectasia Carriers.

Descriptive Note : Annual rept. 15 Aug 94-14 Aug 95,

Corporate Author : M D ANDERSON CANCER CENTER HOUSTON TX

Personal Author(s) : Weil, Michael M.

PDF Url : ADA301728

Report Date : 14 SEP 1995

Pagination or Media Count : 9

Abstract : This proposal seeks to determine if the ataxia-telangiectasia (AT) gene acts as a tumor suppressor gene in a subset of breast cancers by looking for tumor loss of constitutional heterozygosity at chromosomal region 11q22-q23, the map position of the AT gene(s). The technical aims are to analyze 300 matched pairs of tumor and normal tissue from breast cancer patients for tumor loss of constitutional heterozygosity using a panel of amplification polymorphisms mapping to 11q22-q23 and, when loss of heterozygosity is detected, to map the extent of that loss. Over the past year, 37 sets of tumor and normal tissue in pathology storage as paraffin embedded samples were screened for 14 polymorphic loci based on simple sequence repeats (SSRs) using the polymerase chain reaction (PCR). Seventeen of these tumors were found to have lost heterozygosity either in the region of the AT gene or an adjacent region that might overlap. All but one of these deletions appear to be interstitial.

Descriptors :   *GENES, *ATAXIA, *BREAST CANCER, POSITION(LOCATION), POLYMERIZATION, NEOPLASMS, REGIONS, SEQUENCES, ALKANES, SAMPLING, EMBEDDING, MAPS, LOSSES, MAPPING, OVERLAP, PATHOLOGY, PATIENTS, AMPLIFICATION, STORAGE, CHAIN REACTIONS, POLYMORPHISM, MAMMARY GLANDS, SUPPRESSORS.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE