Accession Number : ADA302124

Title :   Int-3 Oncogene in Normal and Neoplastic Breast Development.

Descriptive Note : Annual rept. 1 Sep 94-31 Aug 95,

Corporate Author : COLUMBIA UNIV NEW YORK

Personal Author(s) : Kitajewski, Jan

PDF Url : ADA302124

Report Date : SEP 1995

Pagination or Media Count : 20

Abstract : Mutational activation of the murine int-3 oncogene can contribute to mammary gland tumorigenesis. Evidence suggests that int-3 regulates the morphogenesis and functional differentiation of the mouse mammary gland. The proposed research investigates the role of int-3 proteins in the normal physiology of braast development and the biochemical properties critical for mt-3 transforming activity. cDNAs encoding the truncated int-3 oncogene were cloned. A full length cDNA of approximately 7.3 kilobases was subsequently cloned and has been sequenced. The deduced amino acid sequence verifies that int-3 is a Notch/lin-12 family member. Structually, the int-3 protein contains approximately 20 EGF-like repeats, 3 Notch/lin-12 repeats, a transmembrane domain, and an intracellular domain containing six ankyrin repeats. Analysis of int-3 mRNA shows expression in several adult tissues and during mouse embryonic development. Cell lines have been generated that express an epitope-tagged int-3 oncoprotein, which was detected by Western blot and immunofluorescence analysis. Activated int-3 oncoproteins were found primarily in the nucleus and putative nuclear localization signals have been identified. Finally, bacterially produced int-3 proteins have been purified and used to generate rabbit polyclonal serum.

Descriptors :   *NEOPLASMS, *MAMMARY GLANDS, *ONCOGENIC VIRUSES, *BREAST CANCER, TISSUES(BIOLOGY), PRODUCTION, BIOCHEMISTRY, SEQUENCES, LENGTH, CLONES, PHYSIOLOGY, MICE, CELLS(BIOLOGY), ADULTS, AMINO ACIDS, MORPHOGENESIS.

Subject Categories : Medicine and Medical Research
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE