Accession Number : ADA302152

Title :   Lewis Y Antigen as a Target for Breast Cancer Therapy.

Descriptive Note : Annual rept. 1 Sep 94-31 Aug 95,

Corporate Author : WISTAR INST OF ANATOMY AND BIOLOGY PHILADELPHIA PA

Personal Author(s) : Keiber-Emmons, Thomas

PDF Url : ADA302152

Report Date : SEP 1995

Pagination or Media Count : 65

Abstract : Lewis antigens are blood group carbohydrate antigens implicated as potential target antigens in breast cancer. Studies supported by this grant are intended to develop reagents that better target these antigens which might prove to be valuable as immunotherapeutics for breast cancer treatrnent. In the past year, we concentrated on defining recognition properties of anti-Lewis Y antibodies that confer their specificity for Lewis Y. We obseeved important differences among cancer cells in their mode of interaction with endothelium based on carbohydrate-antibody interactions, which strongly suggest a non-selectin mediated pathway for some breast adenocarcinoma adherence. We expanded our recognition studies to identify peptides on phage display libraries that mimic the Y antigen. We have shown that immunization with such peptides induce anticarbohydrate immune responses in mice that react selectively with human breast adenocarcinoma cells. We found that this sera and monoclonals are also cross-reactive with carbohydrate structures on HIV, inhibiting cell flee HIV infection of target cells in vitro. The development of peptides that mimic carbohydrates represents a novel approach that may lead to a new type of therapeutic agent, as well as an agent that is generally useful.

Descriptors :   *ANTIBODIES, *MONOCLONAL ANTIBODIES, *CARBOHYDRATES, *ANTIGENS, *BLOOD GROUPS, *BREAST CANCER, INTERACTIONS, PEPTIDES, TARGETS, IN VITRO ANALYSIS, DISPLAY SYSTEMS, THERAPY, RECOGNITION, MOLECULAR STRUCTURE, RESPONSE(BIOLOGY), MICE, INFECTIOUS DISEASES, IMMUNITY, CELLS(BIOLOGY), LIBRARIES, IMMUNIZATION, MAMMARY GLANDS, LEWISITE, ENDOTHELIUM, BACTERIOPHAGES.

Subject Categories : Pharmacology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE