Accession Number : ADA302258

Title :   Genetic Immunization for Lentiviral Immunodeficiency Virus Infection and Disease.

Descriptive Note : Annual rept.,

Corporate Author : CALIFORNIA UNIV DAVIS

Personal Author(s) : Gardner, Murray B. ; Malone, R. W. ; Torres, J. V. ; McChesney, M. B. ; Luciw, P. A.

PDF Url : ADA302258

Report Date : 30 AUG 1995

Pagination or Media Count : 68

Abstract : In year one of the current project, experiments have been performed to: (1) Examine the "normal" progression of SIV infection and disease using standardized conditions and the various assays proposed for examination of animals receiving DNA vaccines (including virus load, cellular and humoral immune responses, and cytokine profiles). Standardization of our SIV macaque animal model system was performed in preparation for genetic immunization versus SIV immunization. Assays for viral load, antiviral and cellular immune responses and cytokine levels (to SIV and nonspecific antigens) were applied to four SIV infected and two uninfected rhesus macaques. Considerable innate individual animal variation was observed in immune responsiveness to SIV and other antigens, as was corresponding variation in ability to control SIV infection. Our laboratory assays also revealed, somewhat unexpectedly, problems which now largely been resolved. (2) Develop novel expression vectors intended to improve levels of expression of SIV proteins from DNA injection in mice and primates. A number of novel expression vectors have been designed and tested in cell culture and in animals. These expression vector constructs were found to be very active relative to previously available constructions, and are now being adapted for the expression of a variety of SIV envelope proteins.

Descriptors :   *DEOXYRIBONUCLEIC ACIDS, *RESPONSE(BIOLOGY), *INFECTIOUS DISEASES, *HUMAN IMMUNODEFICIENCY VIRUSES, *VACCINES, CONTROL, INJECTION, PROTEINS, ANIMALS, LABORATORIES, PRIMATES, MICE, ANTIVIRAL AGENTS, VIRUSES, IMMUNITY, CELLS(BIOLOGY), ASSAYING, ENVELOPE(SPACE), GENETICS, ANTIGENS, CULTURES(BIOLOGY), HUMORAL IMMUNITY, RHESUS MONKEYS, MACAQUE MONKEYS.

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE