Accession Number : ADA302285

Title :   A Novel 8OKDA Matrix-Degrading Proteinase in Breast Cancer Invasion and Metastasis.

Descriptive Note : Annual rept. 1 Sep 94-31 Aug 95,

Corporate Author : LONG ISLAND JEWISH-HILLSIDE MEDICAL CENTER NEW HYDE PARK NY

Personal Author(s) : Shi, Yuenian E.

PDF Url : ADA302285

Report Date : SEP 1995

Pagination or Media Count : 25

Abstract : (1) Using T47D cells, our lab built a system in which transfection of FGF4 into this cell line allows cells to form progressively growing tumors in athymic nude mice. The overexpression of FGF also forms lymph node metastasis. Both estrogen and progesterone stimulate the tumor growth. This hormone responsible T47D-derived tumorigenic and metastatic model will be valuable in evaluation of the biological relevance of 80kDa proteinase in breast cancer progression. (2). We recently cloned an apparently new TIMP (tissue inhibitor of metalloproteinase) named TIMP4 and examined its expression in normal, benign and malignant human breast tissues. Our results demonstrated an inverse correlation between the TIMP4 expression and breast cancer progression. In addition, in situ hybridization detected the TIMP4 transcript only in the stromal cells of normal and benign breast tissue but not in the stromals of infiltrating breast carcinomas. Our data suggest that the decreased levels of TIMP4 seen in the breast carcinomas may be too low to prevent MIAP activity and cellular invasion. We hypothesize that the down-regulation of TIMP4 contributes human breast cancer progression and correlate directly with clinical aggressiveness of breast cancer.

Descriptors :   *PROTEINS, *NEOPLASMS, *CELLS(BIOLOGY), *MAMMARY GLANDS, *GROWTH(PHYSIOLOGY), *BREAST CANCER, TISSUES(BIOLOGY), MODELS, CORRELATION, CLONES, INVERSION, MICE, HYBRIDIZATION, PROGESTERONE, ESTROGENS, METASTASIS.

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE