Accession Number : ADA302477

Title :   PH Regulation by Breast Cancer Cells In Vitro and In Vivo.

Descriptive Note : Annual rept. 15 Aug 94-14 Aug 95,

Corporate Author : ARIZONA UNIV TUCSON

Personal Author(s) : Gillies, Robert J.

PDF Url : ADA302477

Report Date : 29 SEP 1995

Pagination or Media Count : 51

Abstract : PH is a fundamental parameter of tumor progression. We are investigating the mechanisms regulating both the intracellular and extracellular pH of breast cancer tumors, both in vivo and in vitro. For extracellular pH studies, we are using 31P-NMR spectroscopy of human breast cancer tumors grown in nude mice. In these studies, extracellular pH is measured using an exogenous compound, 3-aminopropylphosphonate (3-APP), and intracellular pH is measured using endogenous inorganic phosphate. Over the past year, we have been refining these techniques, which are now in use by at least three other labs in the world. For measurement of intracellular pH, we are using fluorescence spectroscopy and molecular biological techniques to characterize the activity of plasma membrane associated Vacuolar type H+-ATPase (pV-ATPase). This pump is highly active in approximately half of the tumor cell lines investigated, and correlates highly with both invasive potential and drug resistance. We hypothesize that these are related and can be explained by a rapid rate of endomembrane recycling.

Descriptors :   *IN VITRO ANALYSIS, *IN VIVO ANALYSIS, *CELLS(BIOLOGY), *PH FACTOR, *BREAST CANCER, CONTROL, MEASUREMENT, RECYCLED MATERIALS, FLUORESCENCE, HIGH RATE, BIOLOGY, SPECTROSCOPY, HUMANS, MOLECULES, PARAMETERS, NEOPLASMS, MEMBRANES, MICE, DRUGS, AMINO ACIDS, MAMMARY GLANDS, RESISTANCE(BIOLOGY).

Subject Categories : Medicine and Medical Research
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE