Accession Number : ADA302601

Title :   Cloning Human Chromosome 17 Genes:

Descriptive Note : Annual rept. 15 Sep 94-14 Sep 95,

Corporate Author : BAYLOR COLL OF MEDICINE HOUSTON TX

Personal Author(s) : Lee, Cheng-Chi

PDF Url : ADA302601

Report Date : OCT 1995

Pagination or Media Count : 15

Abstract : Our research interest is focused on the development of new strategies to identify genes from chromosome 17. The isolation of genes transcribed from chromosome 17 will provide candidates for the proposed sporadic breast and ovarian cancer genes. We have recently reported a method for the isolation of chromosome specific cDNAs using high density arrayed cDNA and chromosome specific cosmid libraries. The ability to isolate genes in a chromosome specific manner provides simultaneous identification of the expressed sequence and a chromosomal location. This new technology identifies expressed sequences by reciprocal probing of arrayed cDNA libraries and a chromosome specific cosmid library. We have used these resources to identify 1794 clones from the Los Mamos chromosome 17 cosmid library using probes generated from a placental cDNA library. So far, we have isolated and characterized 42 cDNAs to chromosome 17. Several of these cDNAs mapped to the region 17p13, while 14 other cDNAs mapped to the region of 17q12-22. Recent studies have demonstrated that in some sporadic breast cancer there are loss of heterozygosity (LOH) in these regions of human chromosome 17.

Descriptors :   *CHROMOSOMES, *GENES, *CLONES, *MAMMARY GLANDS, *BREAST CANCER, POSITION(LOCATION), STRATEGY, ISOLATION, DEOXYRIBONUCLEIC ACIDS, IDENTIFICATION, SYNCHRONISM.

Subject Categories : Medicine and Medical Research
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE