Accession Number : ADA302739

Title :   Canine Vocal Fold Fibroblasts in Culture: Expression of alpha-Smooth Muscle Actin and Modulation of Elastin Synthesis.

Descriptive Note : Rept. for 1 Feb 87-31 Jan 91,

Corporate Author : VANDERBILT UNIV NASHVILLE TN

Personal Author(s) : Broadley, Caroline ; Gonzalez, Debra A. ; Nair, Rhada ; Davidson, Jeffrey M.

PDF Url : ADA302739

Report Date : 1991

Pagination or Media Count : 23

Abstract : Fibroblasts from canine vocal fold tissue have been isolated and maintained in culture. These cells express vimentin and a-smooth muscle actin, the characteristic marker of myofibroblast differentiation. Elastin production by vocal fold fibroblasts was compared to that of skin fibroblasts. The effects of transforming growth factor-Beta and hydrocortisone on elastin production were examined. Vocal fold fibroblasts in culture have a high elastogenic capacity compared to skin fibroblasts, and their elastin production can be enhanced by hydrocortisone (1.3uM) and TCF-Beta (10ng/ml). We conclude that the cells within the vocal fold are myofibroblastic in nature, and have high elastogenic potential in vitro. We suggest that connective tissue remodelling may be a continuous process in the vocal fold.

Descriptors :   *FIBROBLASTS, *SKIN(ANATOMY), *DOGS, *GROWTH(PHYSIOLOGY), PRODUCTION, SYNTHESIS, IN VITRO ANALYSIS, MODULATION, CONTINUOUS PROCESSING, ALBUMINOIDS.

Subject Categories : Medicine and Medical Research
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE