Accession Number : ADA302891

Title :   Cellular and Molecular Mechanisms of High Pressure Inotropy in Cardiac Muscle.

Descriptive Note : Final technical rept. 1 Aug 91-31 May 95,

Corporate Author : STATE UNIV OF NEW YORK AT BUFFALO DEPT OF PHYSIOLOGY

Personal Author(s) : Hogan, Perry M. ; Besch, Stephen R.

PDF Url : ADA302891

Report Date : 04 DEC 1995

Pagination or Media Count : 4

Abstract : The objective of the project was to exploit isolated cardiac myocytes as a model system for investigating the effects of hydrostatic pressure on calcium regulated phenomena. This involved 5 specific aims: (1) to develop instrumentation to perform needed measurements of intracellular calcium transients jrf at high hydrostatic pressure and with high time-resolution; and to determine (2) if pressure acts to change resting and/or dynamic cytosolic-calciunrin-the mammallan cardiac myocyte, (3) the role of the sarcoplasmic reticulum in pressure induced inotropy, (4) the role of sodium-potassium pump inhibition in pressure induced inotropy, (5) the role of the sodium-calcium exchanger in pressure induced inotropy. Objective 1 was met with the development of the devices and techniques developed over the course of this project. Objective 2 was met with the initial design of the pressure chamber. Further refinements were required to meet the remaining objectives, which are currently being investigated.

Descriptors :   *CALCIUM, *HIGH PRESSURE, *MUSCLES, *HYDROSTATIC PRESSURE, *CARDIAC ARREST, TRANSIENTS, MODELS, PRESSURE VESSELS, CELLS(BIOLOGY), MOLECULAR PROPERTIES, CHAMBERS, HEART, CYTOLOGY.

Subject Categories : Anatomy and Physiology
      Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE