Accession Number : ADA303143

Title :   Regulation of the Gl-S-Phase Transition in Non-Transformed and Transformed Human Breast Epithelial Cells.

Descriptive Note : Annual rept. 30 Sep 94-29 Sep 95,

Corporate Author : MICHIGAN STATE UNIV EAST LANSING

Personal Author(s) : Conrad, Samuel E.

PDF Url : ADA303143

Report Date : OCT 1995

Pagination or Media Count : 16

Abstract : The goal of this work is to study the regulation of the thymidine kinase (TK) gene in non-tumorigenic and tumorigenic human breast epithelial cells. Previous studies in our laboratory have shown that this gene is regulated during the cell cycle by both transcriptional and post-transcriptional mechanisms. The approach we are using in these studies is to examine the expression of hybrid genes in stably transfected breast cancer cells. Two hybrid genes have been constructed thus far: (1) TK-Luc, which contains the human TK promoter linked to a luciferase reporter gene, will be used to study transcriptional regulation; (2) CMV-TK*, which contains an epitope-tagged human TK cDNA expressed from a constitutive CMV promoter, will be used to study post-transcriptional regulation. These constructs have been transfected into non-tumorigenic (184B5) and tumorigenic (MCF-7) human breast epithelial cells. We are currently in the process of isolating and characterizing stably transfected cell lines. These transfectants will then be analyzed for regulation of the transfected gene(s). The results of these studies will identify the mechanism(s) that regulate cell cycle specific gene expression in normal breast epithelial cells, and establish whether these mechanisms are disrupted during the process

Descriptors :   *EPITHELIUM, *CELLS(BIOLOGY), *MAMMARY GLANDS, *THYMIDINES, *BREAST CANCER, HUMANS, CYCLES, HYBRID SYSTEMS, GENES, CANCER, LUCIFERASE.

Subject Categories : Medicine and Medical Research
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE