Accession Number : ADA303163

Title :   Regulation and Mechanism of Action of the C-MYC Proto-Oncogene in Human Breast Cancer.

Descriptive Note : Annual rept. 1 Oct 94-30 Sep 95,

Corporate Author : COLUMBIA UNIV NEW YORK

Personal Author(s) : Calame, Kathryn

PDF Url : ADA303163

Report Date : OCT 1995

Pagination or Media Count : 31

Abstract : The biological relevance of the c-Myc/YY1 association has been established by demonstrating that the two proteins associate in vivo and showing that the amount of YY1 associated with c-Myc varies when c-Myc levels change. We have also shown that association with c-Myc does not inhibit the ability of YY1 to bind DNA, but appears to alter its ability to associate with basal transcription proteins TBP and TF-IIB. Steady-state c-myc RNA in MCF-7 cells was shown to increase approximately 1OX in response to estrogen. We expect that at least part this effect depends on transcription because we have identified two estrogen-dependent DNase I hypersensitive sites in the c-myc gene. One of these maps to a region containing NF-kB and C/EBP sites. We are exploring how these proteins may mediate a response to estrogen. Finally, we have shown that PRDIBFI/Blimp-1 is a repressor of c-myc transcription. We are exploring its mechanism of action and determining how overexpression of the gene may alter the growth phenotype of various breast tumor cell lines.

Descriptors :   *IN VIVO ANALYSIS, *MAMMARY GLANDS, *ONCOGENIC VIRUSES, *BREAST CANCER, HUMANS, PROTEINS, DEOXYRIBONUCLEIC ACIDS, RESPONSE, RIBONUCLEIC ACIDS, LIQUID OXYGEN, ESTROGENS.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE