Accession Number : ADA303190

Title :   Regulation of Estrogen Receptor Transcription in Breast Carcinoma.

Descriptive Note : Annual rept. 1 Oct 94-30 Sep 95,

Corporate Author : STANFORD UNIV CA

Personal Author(s) : Weigel, Ronald J.

PDF Url : ADA303190

Report Date : OCT 1995

Pagination or Media Count : 36

Abstract : An important transcriptional regulatory element of the estrogen receptor (ER) gene has been identified that binds a protein expressed in ER-positive breast carcinoma. Using a transient expression assay, a seventy-five base pair region of the 5' untranslated leader of the ER gene was identified which augments expression from the ER promoter.This region contains two binding sites for a protein, estrogen receptor factor-1 (ERF-1), which is expressed in ER-positive breast carcinomas. A concentrated ERF-1 binding site probe identified a 30,000 dalton protein. Low level ERF-1 expression was detected in normal human mammary epithelial cells. Abundant ERF-l expression was also found in endometrial carcinoma cell lines that express the ER-positive phenotype. These results indicate that ERF- 1 expression represents a common mechanism of ER regulation in hormonally responsive carcinomas.

Descriptors :   *NEOPLASMS, *CARCINOGENS, *MAMMARY GLANDS, *ESTROGENS, *BREAST CANCER, CONTROL, TRANSIENTS, PROTEINS, BINDERS, ASSAYING, SENSE ORGANS, CANCER.

Subject Categories : Medicine and Medical Research
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE