Accession Number : ADA303248

Title :   Characterization of Breast Cancer Progression by Analysis of Genetic Markers.

Descriptive Note : Annual rept. 30 Sep 94-29 Sep 95,

Corporate Author : AMERICAN REGISTRY OF PATHOLOGY WASHINGTON DC

Personal Author(s) : Lichy, Jack

PDF Url : ADA303248

Report Date : OCT 1995

Pagination or Media Count : 9

Abstract : Genetic changes implicated in the etiology of breast cancer have been identified by the detection of loss of heterozygosity at specific loci. Our study utilizes a series of genetic polymorphisms detectable by the polymerase chain reaction (PCR) to look for changing patterns of LOH as breast cancer progresses from intraductal to metastatic disease. The initial phases of this work involved the identification of cases from the AFIP archives, microdissection of individual tumor components, and PCR amplification with multiple markers. To date, 248 cases have been identified for the study. Lysates have been prepared from 93 cases. Oligonucleotides were synthesized to detect the nine polymorphisms targeted for the initial screen in the grant proposal. Additional markers were identified and tested to determine ones that would be useful for the study. The 93 cases for which lysates have thus far been prepared have been analyzed for LoH at two loci on chromosome lip15. The results demonstrate our ability to evaluate individual tumor components for LOH and validate the general approach, which will be used extensively in the next phase of the project.

Descriptors :   *HETEROGENEITY, *GENETICS, *GENETIC MAPPING, *MAMMARY GLANDS, *METASTASIS, DETECTION, POLYMERIZATION, DISEASES, NEOPLASMS, IDENTIFICATION, ETIOLOGY, MARKERS, LOSSES, STATICS, CHAIN REACTIONS, POLYMORPHISM, CANCER, BREAST CANCER.

Subject Categories : Medicine and Medical Research
      Genetic Engineering and Molecular Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE