Accession Number : ADA303372

Title :   A New Immunologic Method for Detection of Occult Breast Cancer.

Descriptive Note : Annual rept. 1 Oct 94-30 Sep 95,

Corporate Author : JOHN WAYNE INST FOR CANCER TREATMENT AND RESEARCH SANTA MONICA CA

Personal Author(s) : Gupta, Rishab K.

PDF Url : ADA303372

Report Date : OCT 1995

Pagination or Media Count : 21

Abstract : We investigated the incidence of a 90kD subunit containing glycoprotein tumor-associated antigen (TAA)- specific immune complexes (IC) in sera of breast cancer patients and correlated it with other tumor markers - CEA and CAi5-3. Sera were obtained from 106 patients with histopathologically proven breast cancer, and from 107 self-proclaimed healthy females. Serum samples were analyzed for the presence of the 90kD glycoprotein TAA-specific IC by a murine monoclonal antibody, ADl-40F4, based ELISA. The antibody, developed against an autoimmunogenic glycoprotein TAA, recognized an epitope that is different from those recognized by human polyclonal antibodies. IC captured by AD1-40F4 were realized by enzyme conjugated goat anti-human IgG. A positive test corresponded to a normalized ELISA value above 0.410 OD (mean plus 3 SD at 405nm). The incidence of 90kD glycoprotein TAA-specific IC was significantly higher (p<0.05) in breast cancer patients (63%, or 67/106) than in healthy controls (2.8%, or 3/107). Comparison of the glycoprotein TAA-specific IC results in breast cancer patients with evidence of disease with results of CEA and CA 15-3 revealed that the incidence of abnormal values was increased to 91%. Thus, use of the glycoprotein TAA-specific IC marker in conjunction with CEA and/or CA15-3 may prove to be more sensitive than when used alone for immunodiagnosis and immunoprognosis.

Descriptors :   *GLYCOPROTEINS, *DIAGNOSIS(MEDICINE), *IMMUNOLOGY, *MAMMARY GLANDS, *BREAST CANCER, TEST AND EVALUATION, DETECTION, ENZYMES, NEOPLASMS, SAMPLING, MARKERS, PATIENTS, MONOCLONAL ANTIBODIES, BLOOD SERUM, IMMUNITY, IMMUNOASSAY, GOATS, FEMALES, ABNORMALITIES, CANCER.

Subject Categories : Medicine and Medical Research
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE