Accession Number : ADA303469

Title :   Line-1 Retrotransposons as Mutagens in Human Breast Cancer.

Descriptive Note : Annual rept. 30 Sep 94-29 Sep 95,

Corporate Author : ARMED FORCES INST OF PATHOLOGY WASHINGTON DC

Personal Author(s) : Fanning, Thomas G.

PDF Url : ADA303469

Report Date : OCT 1995

Pagination or Media Count : 8

Abstract : The human LIAE-1 retrotransposon (L1Hs) is known to cause mutations by inserting into genes and inactivating them. The element is expressed in many breast tumors and breast tumor cell lines, suggesting that L1Hs-induced mutations may play some role in this malignancy. To test this hypothesis we plan to place a 'tagged' L1Hs element in non-malignant breast epithelial cells and let it transpose. If the cells become malignant, we can isolate the gene(s) into which the element has transposed by using the unique tag. To date we have identified an appropriate cell line, MCF1OA, but attempts to obtain stably transfected cells expressing L1Hs-encoded proteins have been a failure. We are currently exploring the use of other vectors that may increase our chance of success.

Descriptors :   *GENES, *MAMMARY GLANDS, *BREAST CANCER, HUMANS, CELLS, NEOPLASMS, MUTATIONS, HYPOTHESES, MUTAGENS, CANCER.

Subject Categories : Medicine and Medical Research
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE