Accession Number : ADA303512

Title :   The Role of G2/M Checkpoint Controls in Cytotoxic Treatment of Breast Cancer.

Descriptive Note : Annual rept. 15 Aug 94-14 Aug 95,

Corporate Author : SCRIPPS RESEARCH INST LA JOLLA CA

Personal Author(s) : McGowan, Clare H.

PDF Url : ADA303512

Report Date : 14 AUG 1995

Pagination or Media Count : 11

Abstract : The experiments described are based on the hypothesis that the development and progression of breast cancer is associated with alterations in the operation of cell-cycle checkpoints that normally maintain the fidelity and coordination of cell division and that these alterations determine the susceptibility of cells to cytotoxic therapy This research is intended to provide a detailed understanding of the action of cytotoxic anti-cancer agents and therefore addresses the issue of how treatment of breast cancer can be improved. Using the techniques of biochemistry and molecular biology we have examined the pattern of expression and activity of mitotic checkpoint control proteins (CDC2, Cyclin B, Cyclin A) in transformed and non-transformed breast cells. These results suggested that would be necessary to directly control the level of expression of CDC2,Cyclin B,WEEl and CDC25 (by transfection) in order to determine the importance of checkpoint control proteins in cytotoxic sensitivity. These experiments, which are expected to provide evidence of a causal relationship between changes in cell cycle control proteins and the efficacy of cytotoxic therapy, are currently in progress when - these experiments are completed we will examine the response of transformed and non-transformed breast cell lines to cytotoxic treatments.

Descriptors :   *CYTOTOXINS, *BREAST CANCER, CONTROL, CELLS, BIOCHEMISTRY, PROTEINS, SENSITIVITY, CYCLES, PATTERNS, THERAPY, MOLECULAR BIOLOGY, CELL DIVISION, HYPOTHESES, CHEMOTHERAPY, CANCER, MAMMARY GLANDS.

Subject Categories : Medicine and Medical Research
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE