Accession Number : ADA303836

Title :   Genetic Toxicity Evaluation of 1,3,3-Trinitroazetidine. Volume 3. Results of Gene Mutation at the HGPRT Locus in Cultured Chinese Hamster Ovary Cells.

Descriptive Note : Final rept. Jul-9 Dec 92,

Corporate Author : TOXIKON CORP WOBURN MA

Personal Author(s) : Paika, Inder J.

PDF Url : ADA303836

Report Date : FEB 1994

Pagination or Media Count : 28

Abstract : The test substance, 1, 3,3-Trinitroazetidine (TNAZ)both in the presence and absence of microsomal S-9 liver enzyme, failed to induce a significant increase in mutant colonies at the HGPRT locus in cultured Chinese hamster ovary cells. The effects of TNAZ at the dose levels selected based on the initial range finding assay were similar to those of the negative control (incomplete Ham's F 12 medium) and solvent control (dimethylsulfoxide) substances. Both positive control substances (4-nitroquinoline- I-oxide and dimethylnitrosamine) in the presence and absence of an activation system, exhibited a significantly increased induction of mutant colonies. Confirmatory assays were performed utilizing the same test system conditions as the initial assays. The results were similar and consistent with the initial mutagenicity assay. The data indicates that TNAZ did not induce an increased number of mutants compared to the negative and solvent control substance values. Therefore, TNAZ is considered non-mutagenic under the conditions utilized in this study.

Descriptors :   *TOXICITY, *ENZYMES, *MUTATIONS, *LIVER, *CELLS(BIOLOGY), *GENETICS, *OVARIES, TEST AND EVALUATION, CONTROL, ACTIVATION, METHYL RADICALS, DOSAGE, LEVEL(QUANTITY), SOLVENTS, GENES, RANGE FINDING, ASSAYING, COLONIES(BIOLOGY), INDUCTION SYSTEMS, HAMSTERS, CHINA, LOCUS, NITROSAMINES.

Subject Categories : Toxicology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE