Accession Number : ADA303837

Title :   Genetic Toxicity Evaluation of 1,3,3-Trinitroazetidine. Volume 1. Results of Salmonella Typhimurium Reverse Mutation Assay.

Descriptive Note : Final rept. Jul-Dec 92,

Corporate Author : TOXIKON CORP WOBURN MA

Personal Author(s) : Paika, Inder J.

PDF Url : ADA303837

Report Date : FEB 1994

Pagination or Media Count : 24

Abstract : The Salmonella typhimurium reverse mutation assay (Ames assay) evaluated the potential of 1 3,3-trinitroazetidine (TNAZ) to induce histidine (his) reversion (his to his+), caused by base changes or frameshift mutations in the genome of this organism. This direct plate incorporation assay was conducted with five strains of Salmonella typhimurium, in the presence and absence of an exogenous mammalian activation system. The preincubation technique was used to enhance the sensitivity 0 the plate incorporation assay. The range finding study was conducted with and without metabolic activation to determine levels at which TNAZ exhibited toxicity. The reverse mutation assay determined the test substance to be non-mutagenic. The results of the assay were confirmed through an independent confirmatory assay.

Descriptors :   *TOXICITY, *GENETICS, *SALMONELLA TYPHIMURIUM, TEST AND EVALUATION, ACTIVATION, MAMMALS, METABOLISM, MUTATIONS, REVERSIBLE, STRAINS(BIOLOGY), RANGE FINDING, ASSAYING, HISTIDINE.

Subject Categories : Toxicology
      Genetic Engineering and Molecular Biology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE