Accession Number : ADA303838

Title :   Genetic Toxicity Evaluation of 1,3,3-Trinitroazetidine. Volume 4. Summary report on the Genotoxicity of TNAZ.

Descriptive Note : Final rept. Jul 92-Dec 93,

Corporate Author : TOXIKON CORP WOBURN MA

Personal Author(s) : Prezioso, J. A. ; Desai, L. ; Fitzgerald, G. ; Paika, Inder J. ; DiGiulio, N.

PDF Url : ADA303838

Report Date : FEB 1994

Pagination or Media Count : 22

Abstract : In this study, 1,3,3-trinitroazetidine (TNAZ), a potential component of Air Force explosive formulations, was examined for mutagenic and clastogenic activity. The mutagenic activity of TNAZ was evaluated by both the Salmonella Microsome Reverse Mutation Assay and the Chinese Hamster Ovary Cell/Hypoxanthine-Guanine Phosphoribosyl Transferase (CHO/HGPRT) Forward Mutation Assay. TNAZ, in the presence and absence of 5-9 microsomal enzymes, failed to induce histidine reversion (his- to his+) by base changes or frameshift mutation in the genome of five strains of Salmonella typhimurium bacterial. Furthermore, TNAZ, with and without 5-9 metabolic activation, failed to induce a significant increase in mutant colonies at the HGPRT locus in cultured CR0 cells. Additional independent confirmatory Ames and CHO/HGPRT assays indicated TNAZ was non-mutagenic. TNAZ was also examined for its effect on the chromosomes and mitotic apparatus of albino Swiss mice bone marrow cells by measuring micronuclei formation in maturing erythrocytes. The mouse bone marrow micronucleus test measures the clastogenic potential of chemicals in vivo. The number of micronucleated cells in the maturing erythrocytes in the bone marrow cells of mice was not increased in the presence of TNAZ but was increased greater than 30 fold by mitomycin C. In conclusion, under the test systems and conditions employed in this study, TNAZ is considered non-mutagenic and non-clastogenic.

Descriptors :   *TOXICITY, *MUTATIONS, *GENETICS, *SALMONELLA TYPHIMURIUM, TEST AND EVALUATION, AIR FORCE, FORWARD AREAS, CHEMICALS, FORMULATIONS, EXPLOSIVES, CHROMOSOMES, MICE, IN VIVO ANALYSIS, ERYTHROCYTES, CELLS(BIOLOGY), ASSAYING, SALMONELLA, BONE MARROW, COLONIES(BIOLOGY), LOCUS, MITOSIS.

Subject Categories : Toxicology
      Biochemistry
      Genetic Engineering and Molecular Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE