Accession Number : ADA304203

Title :   Multiple Gene Blotting to Screen for Cytokine RNA Expression: Application to Endothelioma Cells.

Descriptive Note : Technical rept. 1992-1994,

Corporate Author : NAVAL MEDICAL RESEARCH INST BETHESDA MD

Personal Author(s) : Chang, Henry ; Li, Ying-Yue ; Siebert, Paul D. ; Rollwagen, Florence M. ; Nielsen, Thor B.

PDF Url : ADA304203

Report Date : DEC 1995

Pagination or Media Count : 12

Abstract : BACKGROUND: Because normal and tumor cells can secrete different cytokines, it has been a matter of educated guesswork to select which factors to study. Nevertheless, many can be missed, so we have developed a method to screen for unanticipated cytokine expression. EXPERIMENTAL DESIGN: Amplification products from polymerase chain reactions (PCR) of different cytokine genes were spotted onto nylon membranes, and probed with radioactive cDNAs made from mouse endothelioma cells. RESULTS: The presence of interleukin-3 was detected, and confirmed by conventional Northern blotting and polymerase chain reaction (PCR). CONCLUSIONS: This method may be useful to survey cells for the expression of cytokine genes.

Descriptors :   *NEOPLASMS, *MEMBRANES, *GENES, *CELLS(BIOLOGY), *CANCER, *ENDOTHELIUM, CELLS, EXPERIMENTAL DESIGN, POLYMERIZATION, NYLON, DEOXYRIBONUCLEIC ACIDS, SURVEYS, AMPLIFICATION, MICE, CHAIN REACTIONS.

Subject Categories : Medicine and Medical Research
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE