Accession Number : ADA304727

Title :   Cell-Cell Adhesion and Breast Cancer.

Descriptive Note : Annual rept. 15 Dec 94-14 Dec 95,

Corporate Author : GEORGETOWN UNIV WASHINGTON DC

Personal Author(s) : Byers, Stephen

PDF Url : ADA304727

Report Date : JAN 1996

Pagination or Media Count : 88

Abstract : The objective of this proposal is to elucidate the role of cell-cell adhesion to the calcium dependent cell adhesion molecule E-cadherin in breast tumor progression. We will test the hypothesis that in addition to the occasional loss of E-cadherin expression, breast tumor progression is more realistically modeled by a loss of strong cell-cell adhesion resulting from defects in any one or more of the steps (molecules) required for E-cadherin function. During the past year we have developed and used novel biophysical techniques to measure cell-cell adhesive strength. These results show that E-cadherin negative tumor cells, or cells in which the adhesion molecule is present but is inefficiently linked to the cytoskeleton by catenins, are far more likely than E-cadherin positive cells to detach from a tumor mass in response to low shear forces, such as those found in a lymphatic vessel or venule. Since a primary route of dissemination of many carcinoma cells is to the local lymph nodes, these results point to a novel mechanism whereby defects in cell-cell adhesion could lead to carcinoma cell dissemination. Other experiments have demonstrated a role for retinoic acid and serine kinase activity in the regulation of cell-cell adhesion strength in breast cancer cells.

Descriptors :   *ADHESION, *CELLS(BIOLOGY), *MAMMARY GLANDS, *METASTASIS, *BREAST CANCER, FIBERS, DISTRIBUTION, MOLECULES, MASS, PROTEINS, NEOPLASMS, HYPOTHESES, LINKAGES, CELL STRUCTURE, BIOPHYSICS, LYMPH NODES, LYMPHATIC SYSTEM.

Subject Categories : Medicine and Medical Research
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE