Accession Number : ADA304774

Title :   In Vivo Footprinting of the pS2 Gene in Human Breast Cancer Cells.

Descriptive Note : Annual rept. 29 Sep 94-28 Sep 95,

Corporate Author : ILLINOIS UNIV AT URBANA

Personal Author(s) : Nardulli, Ann M.

PDF Url : ADA304774

Report Date : OCT 1995

Pagination or Media Count : 25

Abstract : The regulation of the pS2 gene in MCF-7 human breast cancer cells is being examined to delineate changes that occur at the molecular level in the transformation of normal mammary cells into breast cancer cells. Although the pS2 gene is expressed in breast cancer cells and is subject to regulation by estrogens, it is not expressed in normal mammary cells. By coupling polymerase chain reaction amplification with in vivo footprinting, it is now possible for the first time to examine an endogenous, single copy estrogen-responsive gene residing in normally phased nucleosomes. We will (1) determine if the pS2 estrogen response element is occupied in the absence of hormone, (2) delineate conditions needed for assembly of the basal transcription apparatus, (3) identify regions of the pS2 gene involved in estrogen induced transcription activation, and (4) determine regions of the pS2 gene responsible for mediating the effects of the antiestrogens, trans-hydroxytamoxifen and ICI 164,384. These investigations will enhance our understanding of how hormone-responsive genes are regulated in vivo, identify changes that occur at the molecular level in the progression of mammary cell development to the malignant state, and define how clinically important antiestrogens modulate transcription of estrogen-responsive genes.

Descriptors :   *GENES, *IN VIVO ANALYSIS, *CELLS(BIOLOGY), *MAMMARY GLANDS, *BREAST CANCER, ACTIVATION, MOLECULAR STATES, HUMANS, RADAR FOOTPRINT, ESTROGENS.

Subject Categories : Medicine and Medical Research
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE