Accession Number : ADA304789

Title :   Structural Analysis of the Human T-Cell Receptor/HLA-A2/Peptide Complex.

Descriptive Note : Annual rept. 1 Sep 94-31 Aug 95,

Corporate Author : HARVARD UNIV CAMBRIDGE MA

Personal Author(s) : Wiley, Don C. ; Garboczi, David N.

PDF Url : ADA304789

Report Date : 30 SEP 1995

Pagination or Media Count : 12

Abstract : During the first year of the grant, we obtained sufficient amounts of the T-cell receptor (TCR) for crystallization experiments. This was achieved by protein engineering and by protein refolding experiments. The refolded TCR is now readily obtained in large amounts. The refolded TCR binds to its specific peptide/HLA-A2 complex as detected by native polyacrylamide gel electrophoresis. The ternary complex of TCR/HLA-A2/peptide forms large moderately-well diffracting crystals. We have collected a 3.3 Angstrom dataset and are in the process of determining the structure of the complex to the limit of our data. This structural determination of the T-cell receptor/HLA-A2/peptide complex should allow a detailed understanding of this central molecular recognition event in the immune system.

Descriptors :   *STRUCTURAL PROPERTIES, *PEPTIDES, *CRYSTALLIZATION, *T LYMPHOCYTES, *BREAST CANCER, MOLECULES, PROTEINS, STRUCTURAL ANALYSIS, CRYSTALS, X RAYS, ENGINEERING, RECOGNITION, POLYACRYLAMIDES, IMMUNOLOGY, TERNARY COMPOUNDS, IMMUNITY, GELS, DETERMINATION, ELECTROPHORESIS, SENSE ORGANS, MAMMARY GLANDS.

Subject Categories : Medicine and Medical Research
      Biochemistry
      Crystallography

Distribution Statement : APPROVED FOR PUBLIC RELEASE