Accession Number : ADA304795

Title :   Molecular Genetics of Breast Cancer Neoplasia.

Descriptive Note : Annual rept. 29 Sep 94-28 Sep 95,

Corporate Author : YALE UNIV NEW HAVEN CT SCHOOL OF MEDICINE

Personal Author(s) : Ward, David C.

PDF Url : ADA304795

Report Date : OCT 1995

Pagination or Media Count : 27

Abstract : The overall objective of this grant is to develop new multiparametric fluorescence in situ hybridization (M-FISH) techniques for the analyses of chromosomal abnormalities and specific genes implicated in the initiation and progression of breast carcinomas. During year 01, we identified seven distinct fluorophores and developed optical filter sets to discriminate each of these fluorophores. By using different combinations of fluorophores for probe labeling, we have been able to hybridize up to 27 probes similtaneously, each being identified by its unique spectral signature. We have developed a novel method of chromosome karyotyping using M-FISH such that each chromosome is "painted" a different color. This technique is being used to karyotype chromosome preparations from short-term cultures of breast tumor tissue obtained by needle aspiration. Comparative genome hybridization (CGH) is also being used to define genetic changes that occur in breast tumor cells at various stages of malignancy. Extension of M-FISH to fresh, frozen and paraffin-embedded tissues is in progress. Data from these studies will be assessed with other histological, molecular, and immunological information and correlated with tumor site, size, lymph node status, metatasis, therapy and survival, once a statistically significant number of samples have been analyzed.

Descriptors :   *NEOPLASMS, *CELLS(BIOLOGY), *GENETICS, *MAMMARY GLANDS, *BREAST CANCER, TISSUES(BIOLOGY), FLUORESCENCE, SITES, SURVIVAL(GENERAL), CHROMOSOMES, NODES, SAMPLING, THERAPY, MOLECULAR BIOLOGY, GENES, IMMUNOLOGY, ABNORMALITIES, OPTICAL FILTERS, HYBRIDIZATION, SPECTRUM SIGNATURES, ASPIRATORS, NEEDLES, LYMPH.

Subject Categories : Medicine and Medical Research
      Genetic Engineering and Molecular Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE