Accession Number : ADA304896

Title :   Lipid Peroxidation Following in Vitro and in Vivo O2 Exposures.

Descriptive Note : Technical rept. Oct 92-Oct 94,

Corporate Author : NAVAL MEDICAL RESEARCH INST BETHESDA MD

Personal Author(s) : Harabin, A. L. ; Braisted, J. C.

PDF Url : ADA304896

Report Date : NOV 1995

Pagination or Media Count : 33

Abstract : Four indices of lipid peroxidation were studied in rat brain, lung, and plasma to determine if these would be useful markers of progressive oxidative damage. Conjugated dienes, thiobarbituric acid reactive substances, and water and lipid soluble fluorescent products were measured following IN vitro and in vivo O2 exposures. For in vitro studies, homogenized tissues were oxidized non-enzymatically in increasing amounts of O2. For in vivo studies, rats were exposed to normobaric or hyperbaric O2 for a range of times and pressures. In vitro, all 4 indices increased with PO2 of exposure: brain > lung > plasma. In vivo exposures resulted in lower levels than those achieved in vitro. Hyperbaric exposure produced higher levels than normobaric exposures, but fairly long duration and/or high P02 exposures were required for increases in tissues. On the other hand, plasma thiobarbituric acid reactive substances increased after only 6 h of normobaric 02 exposure and remained elevated. Brain lipid peroxidation was detected following normobaric 02, giving evidence of non-pulmonary oxidation. These results show that there is a relationship between severity of O2 exposure and level of lipid peroxidation, but suggest that other markers should also be investigated for earlier changes.

Descriptors :   *LIPIDS, *IN VITRO ANALYSIS, *IN VIVO ANALYSIS, *HYPERBARIC MEDICINE, *DIENES, *HYPERBARIC CONDITIONS, *THIOBARBITURIC ACID, TISSUES(BIOLOGY), FLUORESCENCE, DAMAGE, EXPOSURE(GENERAL), BRAIN, RATS, WATER, PLASMAS(PHYSICS), REACTIVITIES, LONG RANGE(TIME), OXIDATION, LOW LEVEL, LUNG, INDEXES, MARKERS, SOLUBILITY, HOMOGENEITY.

Subject Categories : Anatomy and Physiology
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE