Accession Number : ADA305616

Title :   In Vivo and In Vitro Analysis of the Regulation of c-myc Proto-Oncogene Expression in Human Breast Cancer.

Descriptive Note : Annual rept. 15 Sep 94-14 Sep 95,

Corporate Author : MASSACHUSETTS GENERAL HOSPITAL BOSTON

Personal Author(s) : Imbalzano, Anthony N.

PDF Url : ADA305616

Report Date : OCT 1995

Pagination or Media Count : 69

Abstract : The purpose of this research is to characterize the factors controlling the expression of c-myc and other genes that are commonly mis-regulated in transformed cells, Genomic DNA exists in the form of chromatin, which generally renders the DNA inaccessible to factors that promote gene expression. Here we report characterization of a cellular protein complex, hSW1/SNF, that can alter chromatin structure and facilitate the interaction of transcription factors with chromatin. The results indicate that the change in chromatin structure induced by hSW1/SNF is stable and not transient in nature, that hSW1/SNF activity requires ATP hydrolysis, that the hydrolysis of ATP is likely utilized to alter the chromatin structure as opposed to modifying or altering the conformation of hSW1/SNF, and that the increased ability of transcription factors to bind to chromatin is not dependent on concurrent hSW1/SNF activity, which indicates that hS1/SNF facilitated binding of transcription factors to nucleosomal DNA is a multi-step process. The results also show that hSWI/SNF can increase transcriptional elongation in vitro on a reconstituted nucleosomal human hsp7O promoter, a promoter that, like c-myc, is regulated at the level of transcription initiation and transcription elongation.

Descriptors :   *DEOXYRIBONUCLEIC ACIDS, *GENES, *CHROMATIN, *BREAST CANCER, HUMANS, CELLS, PROTEINS, IN VITRO ANALYSIS, HYDROLYSIS, IN VIVO ANALYSIS, ELONGATION, MAMMARY GLANDS, CONFORMITY.

Subject Categories : Anatomy and Physiology
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE