Accession Number : ADA305837

Title :   Detection and Clinicopathologic Correlation of Human Immunodeficiency Virus (HIV-1) Nucleic Acids and Antigens in Reticuloendothelial and CNS.

Descriptive Note : Final rept. 30 Aug 90-31 Jul 95,

Corporate Author : ARMED FORCES INST OF PATHOLOGY WASHINGTON DC

Personal Author(s) : Burke, Allen P.

PDF Url : ADA305837

Report Date : FEB 1996

Pagination or Media Count : 14

Abstract : We collected at autopsy lymph nodes and tonsils from 50 dmg addicts who were seropositive for HIV-1 but had no evidence of opportunistic infection or HIV-1 symptomatology. There was diffuse mild enlargement of lymph nodes, which showed B-cell hyperplasia and generalized HIV-1 RNA predominantly in a distribution of follicular dendritic cells. Quantitative polymerase chain reaction estimated viral DNA as a ratio of copies of HIV-1 to HER-2 genomic DNA. Mean HIV-1 genomic DNA ratios were 0.004 for FH-FF, 0.03 for FH+FF, 0.09 for FI and 0.08 for LS. In surgically acquired tonsillar tissues from HIV-1 infected patients, many cells with HIV-1 gag protein were found at the surface of the nasopharyngeal tonsil or adenoid. This infected mucosal surface contained T cells and dendritic cells, which are multinucleate syncytia expressing S-lO0 protein and p55 dendritic cell markers. We conclude that there is abundant HIV-1 viral DNA and RNA within solid lymphoid tissue in early stages of infection. The interaction of dendritic cells and T cells in mucosa likely drives HIVC-1 replication even in subclinical stages of infection.

Descriptors :   *INFECTIOUS DISEASES, *HUMAN IMMUNODEFICIENCY VIRUSES, *ANTIGENS, *NUCLEIC ACIDS, *RETICULOENDOTHELIAL SYSTEM, RATIOS, DETECTION, INTERACTIONS, CELLS, PROTEINS, CLINICAL MEDICINE, DEOXYRIBONUCLEIC ACIDS, CORRELATION, MARKERS, PATHOLOGY, T LYMPHOCYTES, CELLS(BIOLOGY), RIBONUCLEIC ACIDS, DENDRITIC STRUCTURE, CENTRAL NERVOUS SYSTEM, AUTOPSY, DRUG USERS, LYMPH NODES, TONSILS.

Subject Categories : Medicine and Medical Research
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE