Accession Number : ADA306447

Title :   Weak Electromagnetic Field Effects on Gene Expression in E. coli.

Descriptive Note : Final rept. Dec 94,

Corporate Author : WISCONSIN UNIV-PARKSIDE KENOSHA BIOMEDICAL RESEARCH INST

Personal Author(s) : Goodman, E. M. ; Greenebaum, B.

PDF Url : ADA306447

Report Date : 12 MAR 1996

Pagination or Media Count : 9

Abstract : We have previously shown that exposing Eschenchia coli to a magnetic fields (MFs) increased the levels of some proteins and decreased others. Using a cell-free system we reported that expression (transcription and/or translation) of the a and BB' subunits of DNA-directed RNA polymerase were elevated following exposure to 45 Hz, 1.1, 0.21 and 0.07 mT magnetic fields. In the last phase of this program, we sought to determine whether transcription and/or translation were altered by MFs. A cell-free E. coli system was used to determine whether or not MFs directly affect the translational or translational processes. Our data suggest that MFs have no significant direct effect on either transcription or translation. In contrast, when a eukaryotic HeLa extract containing a cmyc plasmid was exposed to a magnetic field, enhanced transcription was observed. At this time we conclude that our inability to distinguish differences in transcription and translation in E. coli may be related to the coupled nature of these processes in the bacterial system.

Descriptors :   *ELECTROMAGNETIC FIELDS, *ESCHERICHIA COLI, *GENES, *RIBONUCLEIC ACIDS, MAGNETIC FIELDS, BACTERIA, ELECTROMAGNETIC PROPERTIES, PROTEINS, PLASMIDS, LOW STRENGTH.

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE