Accession Number : ADA306742

Title :   A Genetic Approach to Identifying Signal Transduction Mechanisms Initiated by Receptors for TGF-B-Related Factors.

Descriptive Note : Annual rept. 1 Oct 94-30 Sep 95,

Corporate Author : WISCONSIN UNIV-MADISON

Personal Author(s) : Hoffmann, F. M.

PDF Url : ADA306742

Report Date : OCT 1995

Pagination or Media Count : 8

Abstract : Genetic screens for new mutations affecting the signaling pathway by which cells respond to TGF-beta-related factors in Drosophila were carried out. Two of the genetic strategies employed were unsuccessful and were abandoned. Two other genetic backgrounds that were sensitized using mutations in the Type I receptor gene and in a candidate transcription factor on the signaling pathway have been used successfully to recover new mutations affecting the phenotypes caused by the sensitized genetic backgrounds. The new mutations were recovered at frequencies that are consistent with a limited number of genes (2-3) being present in which mutations can alter the chosen phenotypes. These 10 new mutations are currently being characterized and mapped. Additional genetic screens to recover more mutations are in progress. The genetic mapping of these new mutations will indicate which cloning strategies should be employed for the molecular identification of the affected genes.

Descriptors :   *MUTATIONS, *GENETICS, *GENETIC MAPPING, *DROSOPHILA, *BREAST CANCER, STRATEGY, MOLECULES, IDENTIFICATION, SIGNALS, BACKGROUND, GENES, CLONES, GENETIC ENGINEERING, SENSE ORGANS, SENSITIZING.

Subject Categories : Genetic Engineering and Molecular Biology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE